highly significantP < 0.001
Two-way ANOVA showed a highly significant effect of mean temperature (15 or 25 °C) (F (1,189) = 67.0, P < 0.001) and temperature regime (constant or fluctuating) (F (1,189) = 9.7, P = 0.002), while the interaction among these two factors was non-significant (F (1,189) = 3.4, P = 0.067).