Importantly, the negative control, in which PLA was measured in mock transfected HeLa cells produced only background signals (5.8 ± 0.5 spots/cell, n=59 ) with the calculated differences being highly significant ( Fig 4g ).
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eEF2 and Ras-GAP SH3 domain-binding protein (G3BP1) modulate stress granule assembly during HIV-1 infection.
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