High-throughput sequencing and graph-based cluster analysis facilitate microsatellite development from a highly complex genome.

The difference in the success rate of PALs within singletons and clusters, defined as the proportion generating polymorphic banding patterns consistent with a single locus, was highly significant (29/48 versus 5/48; two‐tailed binomial proportions test, χ 2 = 24.1, df = 1, P < 0.0001).

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