As PTM can alter protein function ( 39 ), the observation that three out of the four GBM samples analyzed contained only the 35 kDa variant (possibly corresponding to NANOG c with some PTMs, as the larger 40 kDa band in the positive 3T3 lysate control is likely to be NANOG a/b) indicates that the types of modified protein present within a tumor may be significant and that the presence of NANOG c in GBM may even be a predictor of tumor aggression.
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