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Increases in Entamoeba histolytica Antibody-Positive Rates in Human Immunodeficiency Virus-Infected and Noninfected Patients in Japan: A 10-Year Hospital-Based Study of 3,514 Patients.

Am J Trop Med Hyg · 2016 · PMC5014266 · PMID 27296390

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an increasing trendno p-value reported
We observed an increasing trend in antibody-positive rates in this large-scale hospital-based study using an IF assay.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.