Quantitative Expression Analysis in Brassica napus by Northern Blot Analysis and Reverse Transcription-Quantitative PCR in a Complex Experimental Setting.

Furthermore when the third factor continuous light was included, the interaction between all three factors was highly significant for normalization with the selection of reference genes (p<0.001), ACT2 alone (p<0.05), but not significant when normalizing with EF1α alone ( S4 Table ).

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