Evaluation of five microbial and four mitochondrial DNA markers for tracking human and pig fecal pollution in freshwater.

The results showed that the concentrations in feces seemed to have a decreasing trend in the order: BacH (10.08 ± 0.82 log 10 copies/g), H-ND6 (9.08 ± 0.58 log 10 copies/g), B.adolescentis (8.97 ± 1.13 log 10 copies/g), HF183 (8.80 ± 1.56 log 10 copies/g), and H-ND5 (8.13 ± 0.57 log 10 copies/g), for the 5 human-associated markers ( Fig. 1A and Table S1 ), and Pig-2-Bac (10.56 ± 0.58 log 10 copies/g), P-CytB (8.96 ± 0.54 log 10 copies/g), L.amylovorus (8.72 ± 0.63 log 10 copies/g), and P-ND5 (7.61 ± 0.57 log 10 copies/g), for the 4 pig-associated ones ( Fig. 1 B and Table S1 ).

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