A longer exposure time of 10 s, followed by quenching, resulted in a K M of 0.69 ± 0.06 mM ( n = 8, p < 0.05), indicating a marginally significant loss of enzyme affinity for its substrate and an upper limit to exposure time.
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Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters.
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