A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a₁, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells.

pastoris expression system may be significant for vaccine development efforts against B. bovis because there are other molecules, such as apical membrane antigen-1 or rhoptry-associated protein-1, that could also be expressed and purified applying this strategy [ 26 – 28 ].

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