Two questions thus arose: 1) why did p38δ-WT predominantly decrease colony numbers in the G418 assay but increase Fluc luminescence, while the effects of p38δ-F324S and p38δ-D176A remained consistent between the two reporters, and 2) what is the cause of decreased Rluc expression in the presence of p38δ? With respect to the first question, it may be significant that variations were most evident in response to p38δ-WT since it, unlike F324S, would be dependent on a network of cellular factors for activation.
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Deciphering fact from artifact when using reporter assays to investigate the roles of host factors on L1 retrotransposition.
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