Importantly, the PIFE values with Bam HI showed a highly significant difference from control reactions with either bovine serum albumin (BSA) or DNA probe with no BamHI site (P values range from 1.2 × 10 −6 to 1.3 × 10 −6 ; two tailed t-test) demonstrating that mwPIFE can reliably detect a protein/NA interaction in a quantitative manner.
← all excerpts
A rapid method for detecting protein-nucleic acid interactions by protein induced fluorescence enhancement.
1
—
—