Identification and validation of differentially expressed transcripts by RNA-sequencing of formalin-fixed, paraffin-embedded (FFPE) lung tissue from patients with Idiopathic Pulmonary Fibrosis.

To determine whether the overlap between the differentially expressed genes in FFPE RNA-Seq and FF microarrays in both datasets was not due to random association, we performed a hypergeometric test which revealed that the overlap was highly significant ( p < 10 -182 ) for both increased and decreased genes.

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