Partial reconstitution of the RNAi response in human cells using Drosophila gene products.

2015 ), overexpression of the wild-type hDcr protein in NoDice/ΔPKR cells expressing the pCD-RLuc dsRNA insert did not induce repression of RLuc expression, when compared to the empty vector negative control, while a highly significant ( P = 0.004) level of inhibition of RLuc expression was detected in cells coexpressing the N1hDcr mutant, which shows a significantly enhanced ability to generate siRNAs from dsRNA substrates ( Kennedy et al. 2015 ).

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