Critical evaluation of KCNJ3 gene product detection in human breast cancer: mRNA in situ hybridisation is superior to immunohistochemistry.

RNA ISH results correlated positively with IHC results ( p <0.05, r S =0.625; figure 3 B; see also table 1 ), and correlation with microarray data was highly significant ( p <0.001, r S =0.861; figure 3 C). 10.1136/jclinpath-2016-203798.supp5 Supplementary file Discussion Here we report two methods for the detection of KCNJ3 in archived FFPE human breast cancer tissue.

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Spearman's rank correlation analysis resulted in borderline significant correlation for IHC versus ISH ( r S : 0.625; p <0.05) and IHC versus microarray ( r S : 0.668; p <0.01), but in significant correlation for ISH versus microarray ( r S : 0.861; p <0.001).

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