Molecular methods may over-estimate quantity of Leptospira excreted in urine as they detect alive and dead bacteria, however microscopy quantification, detecting live cells shed by rats are within the range detected by qPCR, suggesting that the quantity of dead cells may not be significant.
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Meta-analysis to estimate the load of Leptospira excreted in urine: beyond rats as important sources of transmission in low-income rural communities.
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