Taking tissue expansion into account, we observed that the total fluorescence intensity increased to 1.18 ± 0.11 for 42 °C and 1.08 ± 0.17 for 47 °C, which were comparable to 37 °C, while present highly significant reduction for 52 °C and 57 °C.
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Elevated-temperature-induced acceleration of PACT clearing process of mouse brain tissue.
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