We confirmed this finding by showing that log-transformed translation efficiencies in all three organisms showed highly significant correlations with the predicted degree of mRNA secondary structure (Δ G folding ) in the initiation region (defined here as −30 to +30 nucleotides relative to the first base of the start codon, which was labelled +1; R 2 = 0.13, 0.10 and 0.08 for Escherichia coli , C. crescentus and B. subtilis , p < 10 −42 for all cases).
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Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency.
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