Barely Significant
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Plasma-derived parasitic microRNAs have insufficient concentrations to be used as diagnostic biomarker for detection of Onchocerca volvulus infection or treatment monitoring using LNA-based RT-qPCR.

Parasitol Res · 2017 · PMC5313568 · PMID 28111713

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highly significantP < 0.001actually significant
Even though both sample sets were analyzed using the same qPCR conditions, a highly significant difference in Tm values was appreciated ( P < 0.001), indicating that the signals obtained for these miRNAs in the clinical samples are not specific and are presumably caused by non-specific amplification of one or more other miRNAs present in the samples.

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