The compartment particle density versus compartment area data was fit by a straight line regression and while the slope failed to reach significance ( R 2 = 0.178, p = 0.405) we could nonetheless calculate the 95% Prediction Bands.
Further, analysis of individual tethered SVs exhibited a highly significant elevated nanogold cluster frequency on the tethers and near the AZ membrane, while no clusters were associated with the tethered SVs themselves ( Figure 9A ).
With NmidC2, compartment analysis gold particle frequency was almost significant for AZ puffs, the electron dense ‘clumps’ on the AZ surface membrane ( Phillips et al., 2001 ), but was significantly increased for AZ filaments ( Figure 7B ).