A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3'end using CRISPR-Cas9 genome editing technique.

Again this increase in the NanoLuc luciferase activity complied with an increasing trend in α-SYN/Nanoluc mRNA and protein expression after dopamine treatment as seen by RT-PCR and western blot analyses ( Fig. 3c , Supplementary Fig. 4 ).

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