The evolution of Sex-linked barring alleles in chickens involves both regulatory and coding changes in CDKN2A.

We were able to detect expression of all genes under the described conditions except for BAX , but only PHLDA3 showed a marginally significant higher expression in B0/- feathers (Student’s t-test, P = 0.03; S3 Fig ).

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How is this result compatible with the highly significant allelic imbalance test showing that the mutant allele is expressed at a higher level than the wild-type allele in feather follicles ( Fig 2B )?

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