trend toward significancep = 0.08
Although there was no significant main effect of sex/phase of estrous cycle ( F 3,21 = 0.61, p = 0.62, η G 2 = 0.06), there was an apparent trend toward significance for the interaction between sex/phase of estrous cycle and day ( F 3,21 = 2.56, p = 0.08, η G 2 = 0.07) (Additional file 4 : Figure S4C, D).