Barely Significant
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The abundance of the Lyme disease pathogen Borrelia afzelii declines over time in the tick vector Ixodes ricinus.

Parasit Vectors · 2017 · PMC5445446 · PMID 28545520

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highly significantP < 0.0001actually significant
Effect of nymphal age on the spirochete load of infected nymphs The mean spirochete load of the 1-month-old nymphs ( n = 43, mean: 14,094, 95% confidence interval (CI): 13,508–14,705 spirochetes per nymph) was 7.05 times larger than that of the 4-month-old nymphs ( n = 42, mean: 1999, 95% CI: 1900–2103 spirochetes per nymph) and this difference was highly significant ( t = 12.768, df = 41, P < 0.0001).

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