Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity.

Sci Rep · 2017 · PMC5489508 · PMID 28659635

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a slight trendno p-value reported

2 ), there was a slight trend toward alteration of the bacterial composition of some genera in Firmicutes: with pretreatment there was a decrease in Faecalibacterium in some individuals, a marginal increase in Blautia , but no apparent change in Ruminococcus ( Faecalibacterium , p = 0.421; Blautia , p = 0.661; Ruminococcus , p = 0.839) (Fig. 2 ; Group 2).

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