Therefore, we defined highly significant differentially expressed unigenes (HDEGs) on the basis of the stringent thresholds (i.e., |Log2(ratio)| ≥ 1 and FDR < 0.001 and Max(FPKM) ≥ 100) in this present study.
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Transcriptome analysis explores genes related to shikonin biosynthesis in Lithospermeae plants and provides insights into Boraginales' evolutionary history.
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