First detection and genotyping of Giardia intestinalis in stool samples collected from children in Ghazni Province, eastern Afghanistan and evaluation of the PCR assay in formalin-fixed specimens.

The visible advantage of real-time PCR was most probably due to amplification of a much shorter product (74 bp) than in the case of nested PCR (497 bp in first-round PCR), which may be significant when degradation of DNA occurs in the sample.

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