Immunoassays for the quantification of ALK and phosphorylated ALK support the evaluation of on-target ALK inhibitors in neuroblastoma.

When the levels of phosphorylated ALK in the neuroblastoma cell lines were normalized to total ALK signals from the immunoassays and compared with densitometry of the immunoblots, there was a positive correlation that was statistically significant for the total ALK and pY1278 and pY1586 ALK assays, and although not significant, a positive trend between densitometry and immunoassay for the detection of pY1604 ALK was seen (Fig. 2 C).

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