Exploiting sequence and stability information for directing nanobody stability engineering.

The analysis resulted in a distribution of data points devoid of clearly separated subgroups but indicated a slight trend of increased aliphatic indices and melting temperatures for llama-derived nanobodies ( Fig. 1B ); To reveal common signatures of enhanced protein stability within the nanobody fold, we performed a Global Sequence Signature (GSS) analysis [ 51 ] on a multiple sequence alignment (MSA) constructed from the amino acid sequences of the 78 nanobodies.

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