highly significantp < 0.0001
As shown in Figures 3A,B and Table 2 , while both gO GT1c cysteine mutants, gO GT1c-C218S and gO GT1c-C343S, displayed a similar infectivity for HFFs as their parental strains GT1c_1 and GT1c_2, the mutation of C336S in gO GT4 led to reduction in infectivity, which was significant when compared to GT4_1 ( p = 0.0025) and highly significant when compared to GT4_2 ( p < 0.0001).