A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in <i>Pichia pastoris</i>.

Front Microbiol · 2017 · PMC5601908 · PMID 28955309

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an increasing trendno p-value reported

In test tube culture, the transformants GS115-pMCO-AOXα-An-Zeocin R ( n = 1, 2, 4, or 8) showed an increasing trend in the recombinant protein expression level, and the GS115-pMCO-AOXα-A8-Zeocin R obtained the highest phytase yield (Figure 5A ).

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