We observed a trend toward increased RhoA activation in SK2KO macrophages compared with WT; however, this did not quite reach statistical significance ( Fig. 5A ).
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Ceramide activation of RhoA/Rho kinase impairs actin polymerization during aggregated LDL catabolism.
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WTRhoA-GFP-expressing cells displayed reduced actin polymerization, though this was not quite statistically significant, but CARhoA-GFP-expressing cells displayed significantly reduced actin polymerization specifically at the LS in response to agLDL ( Fig. 6A , arrows, and ).