An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila.

When the frequencies of the four E chromosome arrangements with absolute frequencies ≥ 5 (Table 3 ) were compared between sexes, they did not differ significantly in either case even though frequency differences were marginally significant in the 2015 sample (χ 2 = 3.986; d.f. = 3; P = 0.263; χ 2 = 6.594; d.f. = 3; P = 0.086, in the 2014 and 2015 samples, respectively).

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