Kinetics of CrPV and HCV IRES-mediated eukaryotic translation using single-molecule fluorescence microscopy.

In any case, the departure rate of the R-UP-(+5)-primer is clearly significantly higher with IRES and no cycloheximide ( Fig. 3 A, red curve), and we conclude that this increase in the departure rate is due to translating ribosomes. mRNAs with HCV IRES are also efficiently translated To further confirm these results, we performed the same experiment using an mRNA carrying the HCV IRES with and without a stop codon before the R-DOWN fluorescent primer ( Fig. 3 D).

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