Molecular Cloning of the Vitellogenin Gene and the Effects of Vitellogenin Protein Expression on the Physiology of Harmonia axyridis (Coleoptera: Coccinellidae).

The relative mRNA expression levels of the Vg gene showed markedly significant differences between the treatment groups and the control groups collected on the days 9 ( F = 156.06 6; df = 3, 11; P < 0.001), 18 ( F = 131.693; df = 3, 11; P < 0.001), 26 ( F = 257.967; df = 3, 11; P < 0.001) and 32 ( F = 376.706; df = 3, 11; P < 0.001).The Vg mRNA expression levels in insects treated with 60 μg/mL Vg protein were 27-, 51-, 6-, and 2.5-fold higher than those of control groups on days 9, 18, 26, and 32, respectively.

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