qRT-PCR analysis expression profiling of miRNAs and their targets The expression patterns of nine randomly selected miRNAs using qRT-PCR showed that most stamen miRNAs exhibited a decreasing trend after high-temperature treatment except for miR164a-5p, which was significantly increased at 2 d after high-temperature treatment (Fig. 8 ).
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Identification and expression profiling of microRNAs involved in the stigma exsertion under high-temperature stress in tomato.
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