Vincristine liposomes with smaller particle size have stronger diffusion ability in tumor and improve tumor accumulation of vincristine significantly.

On contrary, the free VCR group and 100 nm VCR-Lip had an extremely significant difference (P<0.01), as well as 30 nm VCR-Lip and 100 nm VCR-Lip.

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“*” mean the P -value less than 0.05 which was considered to be significant and “**” mean the P -value less than 0.01 which was considered to be highly significant.

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The cell inhibition area of free VCR solution, 30 nm VCR-Lip and 100 nm VCR-Lip appears a decreasing trend.

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