Epigallocatechin-3-gallate (EGCG) up-regulates miR-15b expression thus attenuating store operated calcium entry (SOCE) into murine CD4⁺ T cells and human leukaemic T cell lymphoblasts.

As a result, treatment of murine CD4 + T cells with 10 μM EGCG was followed by a marked and highly significant increase of miR-15b abundance (Figure 4 ). miR-15b overexpression (miR-15b mimic transfection) decreases STIM2 and Orai1 both at mRNA transcript and protein levels in activated murine CD4 + T cells Bioinformatics analysis ( http://www.microrna.org ) suggested that murine miR-15b (mmu-miR-15b) could regulate Ca 2+ pathways by regulating STIM1/2 proteins as miR-15b family has a strong binding site in the 3’untranslated region (3’UTR) of STIM2 (Figure 5A ).

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