P ≤ 0.05 was considered statistically significant (*) and P ≤ 0.01 (**), P ≤ 0.001(***), P ≤ 0.0001(****) extremely significant.
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TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes.
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The use of this method confirmed that the accumulation of lysosomes in the cell periphery in TMEM55B-depleted cells is highly significant (Supplementary Fig. 1c ).