Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy.

What was clear was the distinctly different trends for CHX versus Act D, indicating a highly significant change in relative localisation and clustering of genomes in the presence of CHX versus Act D (p-value < 0.0001; D statistic = 0.52).

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With increasing moi there was an increasing trend of genome foci in the nucleus, but this was not directly proportionate, with for example an average of 4–5 foci at moi 10, 6 at moi 20 and 10 at moi 50.

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