almost reached statistical significanceP = 0.0628
We observed no significant changes in the levels of pyridoxine (Pyr), pyridoxamine (PM), 4-pyridoxic acid, and Pyr 5′-phosphate (data not shown), but the null-mutants showed a statistically significant reduction in the levels of pyridoxal (PL, P = 0.0094, Figure 7A ) and pyridoxamine 5′-phosphate (PMP, P = 0.0013, Figure 7B ), and a reduction of PLP that almost reached statistical significance ( P = 0.0628 Figure 7C ) based on a Student’s t -test.