Barely Significant
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Determination of heme in microorganisms using HPLC-MS/MS and cobalt(III) protoporphyrin IX inhibition of heme acquisition in Escherichia coli.

Anal Bioanal Chem · 2017 · PMC5717118 · PMID 29043383

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a weak trendno p-value reported
There was a weak trend that more heme was extracted when longer extraction times were used, but the differences were small and not significant.

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