Repositioning Titanium: An <i>In Vitro</i> Evaluation of Laser-Generated Microporous, Microrough Titanium Templates As a Potential Bridging Interface for Enhanced Osseointegration and Durability of Implants.

A highly significant increase in β-actin expression by day 21 in all three groups was observed, indicating that β-actin expression increased as culture time progressed.

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Although culture time and culture approach were shown to have a significant effect on gene expression in general, the extent of interaction between these two variables was extremely significant for all gene markers tested.

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Given an increasingly aging population worldwide, there has been a rapid expansion of research in the field of biomaterials and scaffold fabrication methods for tissue engineering and regenerative medicine purposes (Hutmacher, 2001 ), with an increasing trend toward utilizing polymeric, ceramic, and/or organic materials (or a combination thereof) to generate biocompatible scaffolds with varying geometry, porosity, and topography (Ducheyne et al., 2017 ).

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