Detection of somatic variants and <i>EGFR</i> mutations in cell-free DNA from non-small cell lung cancer patients by ultra-deep sequencing using the ion ampliseq cancer hotspot panel and droplet digital polymerase chain reaction.

Oncotarget · 2017 · PMC5739783 · PMID 29290998

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borderline significantno p-value reported

In this case, ddPCR could be utilized as a validation test to overcome possible sequencing errors or borderline significant results in targeted NGS ultra-deep sequencing.

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