Efficacy of assessing circulating cell-free DNA using a simple fluorescence assay in patients with triple-negative breast cancer receiving neoadjuvant chemotherapy: a prospective observational study.

In a subgroup with aggressive tumor biology (histologic grade 3, 46 patients), with marginal significance, AC-CFD levels substantially declined in pCR patients than in non-pCR patients, with higher variations seen in CFD levels of pCR patients [AC-CFD levels, pCR (183) vs. non-pCR (219); P = 0.090 and changes in CFD levels, pCR (−62.3) vs. non-pCR (−17.9); P = 0.133; Table 4 ].

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We observed an increasing trend toward higher baseline-CFD levels in patients who relapsed, although it was not statistically significant (relapse, 259 ng/mL; non-relapse, 233 ng/mL; P = 0.161; Table 3 ).

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