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The prevalence of Escherichia coli O157:H7 fecal shedding in feedlot pens is affected by the water-to-cattle ratio: A randomized controlled trial.

PLoS One · 2018 · PMC5802916 · PMID 29414986

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an overall trendno p-value reported
Furthermore, the finding was consistent in years one and two, despite the fact that there was an overall trend of increasing prevalence in year one, from the beginning to the end of the trial, and an overall trend of decreasing prevalence in year two.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.