Interestingly, while the effect of both miRNA deletions on NFκB p65 phosphorylation was highly significant, the same trend was observed for IL1b, IL8 and CCL4 mRNA induction, however did not reach significance levels.
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Cas9-mediated excision of proximal DNaseI/H3K4me3 signatures confers robust silencing of microRNA and long non-coding RNA genes.
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