highly significantp < 0.0001
Two-way ANOVA showed a highly significant treatment effect ( F (1,20) = 47, p < 0.0001), time effect ( F (4,20) = 91, p < 0.0001), and interaction effect ( F (4,20) = 11, p < 0.0001), indicating a time-dependent pharmacological inhibition of DAT functions.