Effects of Beneficial Mutations in pykF Gene Vary over Time and across Replicate Populations in a Long-Term Experiment with Bacteria.

An analysis of variance shows that the fitness effects were largely determined by the population’s genetic background, with the specific mutation having only a marginally significant effect ( table 3 ).

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An ANOVA indicates a highly significant interaction between the population and generation factors ( table 4 , fig. 5 A ), and the interaction remains highly significant if generation 0 is excluded from the analysis ( supplementary table S8 , Supplementary Material online).

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