This is because most studies focused on the particle properties as influenced by different chitosan-TPP mass ratios 7 , 16 , 17 but did not take into account other experimental factors such as the DA of the chitosan used which can be expected to be highly significant in a process that depends on the NH 3 + groups for interaction, like in the case of ionic gelation.
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Parameters influencing the size of chitosan-TPP nano- and microparticles.
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