The Janus of macrophysiology: stronger effects of evolutionary history, but weaker effects of climate on upper thermal limits are reversed for lower thermal limits in ants.

This result for CT min appears unlikely to reflect sampling bias owing to fewer lower tolerance values compared with upper tolerance values (see the Supplementary Material ). Although spatial proximity was not a strong predictor of variation in thermal tolerance (even when restricting our analyses to range centroids occurring at positive latitudes; see the Supplementary Material , Table S1), we found that geographic variation in temperature was a highly significant predictor of variation in both CT max and CT min .

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